Hotspot

APQ78_gt002-APQ78_gp070

Genome · 4188-4742 · 555 bp

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Pi 0.168
MarkerSeek score 77.40
Primer available yes
Label APQ78_gt002-APQ78_gp070

Feature Metadata

label_name
APQ78_gt002-APQ78_gp070
feature_type
igs
region
Genome
length_bp
555
variable_sites
191
pi
0.168
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.82
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gt002-APQ78_gp070_p1 GGAGAACAAAAGCTCGTTGC AAGGCAGGGGTTTATCATCG 50.0 50.0 58.0 57.7 976-986 bp 0.5 60.8
APQ78_gt002-APQ78_gp070_p2 AAAGCCGAGTACTCTACCGT AAGGCAGGGGTTTATCATCG 50.0 50.0 58.2 57.7 664-680 bp 0.5 60.6
APQ78_gt002-APQ78_gp070_p3 AAGCCGAGTACTCTACCGTT AAGGCAGGGGTTTATCATCG 50.0 50.0 58.2 57.7 663-679 bp 0.5 60.6
APQ78_gt002-APQ78_gp070_p4 AGCCGAGTACTCTACCGTTT AAGGCAGGGGTTTATCATCG 50.0 50.0 58.2 57.7 662-678 bp 0.5 60.6
APQ78_gt002-APQ78_gp070_p5 CCGCACTTAAAAGCCGAGTA AAGGCAGGGGTTTATCATCG 50.0 50.0 58.3 57.7 673-689 bp 0.5 59.6