Hotspot

APQ78_gp032

Genome · 60174-117302 · 57129 bp

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Pi 0.0382
MarkerSeek score 71.20
Primer available yes
Label APQ78_gp032

Feature Metadata

label_name
APQ78_gp032
feature_type
gene
region
Genome
length_bp
57129
variable_sites
4701
pi
0.0382
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp032_p1 ACGACCAGTCAGTTAACAGC ACCTATTTCACCATAGCGGC 50.0 50.0 57.8 57.7 877-1040 bp 1.0 88.7
APQ78_gp032_p2 AGGATTCGAACCTACGACCA ACCTATTTCACCATAGCGGC 50.0 50.0 58.2 57.7 890-1053 bp 1.0 88.7
APQ78_gp032_p3 ACGACCAGTCAGTTAACAGC GACTGGAGAGCGGGTACTAA 50.0 55.0 57.8 58.2 1424-1624 bp 0.833333 82.2
APQ78_gp032_p4 AGGATTCGAACCTACGACCA GACTGGAGAGCGGGTACTAA 50.0 55.0 58.2 58.2 1437-1637 bp 0.833333 82.2
APQ78_gp032_p5 GGATTCGAACCTACGACCAG GACTGGAGAGCGGGTACTAA 55.0 55.0 57.8 58.2 1436-1636 bp 0.833333 81.8