Hotspot

APQ78_gp025

Genome · 65604-67031 · 1428 bp

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Pi 0.0501
MarkerSeek score 84.30
Primer available yes
Label APQ78_gp025

Feature Metadata

label_name
APQ78_gp025
feature_type
gene
region
Genome
length_bp
1428
variable_sites
157
pi
0.0501
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp025_p1 TAGCTCCTAGATGGGGAACG GCTTTCTGGGTCGTCTCAAA 55.0 50.0 58.0 58.1 2291-2387 bp 1.0 91.0
APQ78_gp025_p2 TAGCTCCTAGATGGGGAACG CGTTGAGCCCCTTTTCTTCT 55.0 50.0 58.0 58.1 2198-2211 bp 0.333333 53.6
APQ78_gp025_p3 TAGCTCCTAGATGGGGAACG CTCGTTGAGCCCCTTTTCTT 55.0 50.0 58.0 58.1 2200-2213 bp 0.333333 53.6
APQ78_gp025_p4 TAGCTCCTAGATGGGGAACG TCGTTGAGCCCCTTTTCTTC 55.0 50.0 58.0 58.1 2199-2212 bp 0.333333 53.6
APQ78_gp025_p5 TAGCTCCTAGATGGGGAACG ATCTCGTTGAGCCCCTTTTC 55.0 50.0 58.0 57.9 2202-2215 bp 0.333333 53.5