Hotspot

APQ78_gp018

Genome · 71623-73054 · 1432 bp

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Pi 0.0705
MarkerSeek score 84.50
Primer available yes
Label APQ78_gp018

Feature Metadata

label_name
APQ78_gp018
feature_type
gene
region
Genome
length_bp
1432
variable_sites
226
pi
0.0705
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
APQ78_gp018_p1 AATTTTCGAGCCCCGCTATT ACTGAGAGGAATCGACGAGT 45.0 50.0 57.9 57.9 2277 bp 0.166667 45.3
APQ78_gp018_p2 ATTGGTACCCCCGGTGATTA ACTGAGAGGAATCGACGAGT 50.0 50.0 58.1 57.9 2711 bp 0.166667 44.9
APQ78_gp018_p3 TTGGTACCCCCGGTGATTAT ACTGAGAGGAATCGACGAGT 50.0 50.0 58.1 57.9 2710 bp 0.166667 44.9
APQ78_gp018_p4 CTGCCGCATTCTCATCAGAT ACTGAGAGGAATCGACGAGT 50.0 50.0 58.1 57.9 2471 bp 0.166667 44.8
APQ78_gp018_p5 TGCCGCATTCTCATCAGATC ACTGAGAGGAATCGACGAGT 50.0 50.0 58.1 57.9 2470 bp 0.166667 44.7