Hotspot

ycf95

SSC · 125700-126050 · 351 bp

Back to Vischeria

Pi 0.0228

MarkerSeek score 52.60

Primer available yes

Label ycf95

Feature Metadata

label_name: ycf95
feature_type: gene
region: SSC
length_bp: 351
variable_sites: 8
pi: 0.0228
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf95_p1	TTAGTGCGGATGGAGAGACT	AAAACACGCCTTGAAGGACT	50.0	45.0	57.9	57.9	911-928 bp	1.0	80.3
ycf95_p2	TTAGTGCGGATGGAGAGACT	AAACACGCCTTGAAGGACTT	50.0	45.0	57.9	57.9	910-927 bp	1.0	80.3
ycf95_p3	TTAGTGCGGATGGAGAGACT	GAAGGACTTGAACCCTCGAC	50.0	55.0	57.9	57.9	899-916 bp	1.0	80.1
ycf95_p4	TAGTGCGGATGGAGAGACTC	AAAACACGCCTTGAAGGACT	55.0	45.0	58.3	57.9	910-927 bp	1.0	78.9
ycf95_p5	TAGTGCGGATGGAGAGACTC	AAACACGCCTTGAAGGACTT	55.0	45.0	58.3	57.9	909-926 bp	1.0	78.9