Hotspot

rpl27

LSC · 47681-47998 · 318 bp

Back to Vischeria

Pi 0.0377

MarkerSeek score 74.60

Primer available yes

Label rpl27

Feature Metadata

label_name: rpl27
feature_type: gene
region: LSC
length_bp: 318
variable_sites: 12
pi: 0.0377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl27_p1	CGCTAGCATCTTACGGTTCA	TCAAGACGAGTAAGGTTTGACA	50.0	40.9	57.8	57.3	488 bp	1.0	59.1
rpl27_p2	CGCTAGCATCTTACGGTTCA	TCAAGACGAGTAAGGTTTGACAA	50.0	39.1	57.8	58.0	488 bp	1.0	56.2
rpl27_p3	CGCTAGCATCTTACGGTTCA	TTCAAGACGAGTAAGGTTTGACA	50.0	39.1	57.8	58.0	489 bp	1.0	56.2
rpl27_p4	CGCTAGCATCTTACGGTTCA	CAAGACGAGTAAGGTTTGACA	50.0	42.9	57.8	55.9	487 bp	1.0	55.3
rpl27_p5	ACTTTTGGATCAACAACCGC	TCAAGACGAGTAAGGTTTGACA	45.0	40.9	57.2	57.3	514 bp	1.0	54.2