Hotspot

eboC

LSC · 52691-53581 · 891 bp

Back to Vischeria

Pi 0.0393

MarkerSeek score 72.90

Primer available yes

Label eboC

Feature Metadata

label_name: eboC
feature_type: gene
region: LSC
length_bp: 891
variable_sites: 35
pi: 0.0393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
eboC_p1	AGCATATTCAAAATTAACCGTAA	TCAGTGCTAAGAGGCCAAGA	26.1	50.0	52.8	58.4	1033 bp	1.0	42.7
eboC_p2	AGCATATTCAAAATTAACCGTAA	CAGTGCTAAGAGGCCAAGAA	26.1	50.0	52.8	57.5	1032 bp	1.0	42.7
eboC_p3	AGCATATTCAAAATTAACCGTAA	TTCAGTGCTAAGAGGCCAAG	26.1	50.0	52.8	57.5	1034 bp	1.0	42.7
eboC_p4	AGCATATTCAAAATTAACCGTAAA	TCAGTGCTAAGAGGCCAAGA	25.0	50.0	53.6	58.4	1033 bp	1.0	42.7
eboC_p5	ATAGCATATTCAAAATTAACCGT	TCAGTGCTAAGAGGCCAAGA	26.1	50.0	52.6	58.4	1035 bp	1.0	42.7