Hotspot

clpN

SSC · 33787-34251 · 465 bp

Back to Vischeria

Pi 0.0366

MarkerSeek score 62.90

Primer available yes

Label clpN

Feature Metadata

label_name: clpN
feature_type: gene
region: SSC
length_bp: 465
variable_sites: 17
pi: 0.0366
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpN_p1	TGTTCTGGCAACTTTGACCA	ATGCTACACATTCTGCAGCA	45.0	45.0	57.9	57.9	1618-1627 bp	1.0	81.0
clpN_p2	TGTTCTGGCAACTTTGACCA	TGCTACACATTCTGCAGCAT	45.0	45.0	57.9	57.9	1617-1626 bp	1.0	81.0
clpN_p3	TGTTCTGGCAACTTTGACCA	ACAATCCCTGGCTTTGTACG	45.0	50.0	57.9	58.2	1142-1151 bp	1.0	81.0
clpN_p4	CCGATTGCTCTACCACTGAG	ATGCTACACATTCTGCAGCA	55.0	45.0	57.8	57.9	1168 bp	1.0	80.3
clpN_p5	CCGATTGCTCTACCACTGAG	TGCTACACATTCTGCAGCAT	55.0	45.0	57.8	57.9	1167 bp	1.0	80.3