Hotspot

psaI-psbJ

LSC · 104447-104748 · 302 bp

Back to Vischeria

Pi 0.284

MarkerSeek score 73.90

Primer available yes

Label psaI-psbJ

Feature Metadata

label_name: psaI-psbJ
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 85
pi: 0.284
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-psbJ_p1	AGCACGTGCAGAAATAGGTT	CTTGTAGCTTTTGTTGCGGG	45.0	50.0	57.8	57.9	1057-1058 bp	1.0	86.1
psaI-psbJ_p2	AAACCAATGATCGACGAGCA	CTTGTAGCTTTTGTTGCGGG	45.0	50.0	57.9	57.9	1073 bp	0.5	58.8
psaI-psbJ_p3	AAACCAATGATCGACGAGCA	AGACCGTCAACAAGTTCCAC	45.0	50.0	57.9	58.1	1529 bp	0.5	58.8
psaI-psbJ_p4	AAACCAATGATCGACGAGCA	GACCGTCAACAAGTTCCACT	45.0	50.0	57.9	58.1	1528 bp	0.5	58.8
psaI-psbJ_p5	AAACCAATGATCGACGAGCA	CTCAATCCTCATATGGCGGG	45.0	55.0	57.9	57.9	1714 bp	0.5	58.4