Hotspot

petA-thiS

LSC · 91164-91238 · 75 bp

Back to Vischeria

Pi 0.107

MarkerSeek score 74.80

Primer available yes

Label petA-thiS

Feature Metadata

label_name: petA-thiS
feature_type: igs
region: LSC
length_bp: 75
variable_sites: 8
pi: 0.107
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-thiS_p1	GTTCAGGCTGCAGAGATGAA	TGTTACTATAGTTGGGGGTGGT	50.0	45.5	57.9	58.2	127 bp	1.0	66.6
petA-thiS_p2	AAGTTCAGGCTGCAGAGATG	TGTTACTATAGTTGGGGGTGGT	50.0	45.5	57.9	58.2	129 bp	1.0	66.5
petA-thiS_p3	GAAAGTTCAGGCTGCAGAGA	TGTTACTATAGTTGGGGGTGGT	50.0	45.5	57.8	58.2	131 bp	1.0	65.9
petA-thiS_p4	GAGAAAGTTCAGGCTGCAGA	TGTTACTATAGTTGGGGGTGGT	50.0	45.5	57.8	58.2	133 bp	1.0	65.9
petA-thiS_p5	AGAAAGTTCAGGCTGCAGAG	TGTTACTATAGTTGGGGGTGGT	50.0	45.5	57.8	58.2	132 bp	1.0	65.8