Hotspot

ccsA-sufB

LSC · 65101-66042 · 942 bp

Back to Vischeria

Pi 0.0873

MarkerSeek score 88.10

Primer available yes

Label ccsA-sufB

Feature Metadata

label_name: ccsA-sufB
feature_type: igs
region: LSC
length_bp: 942
variable_sites: 80
pi: 0.0873
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 88.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-sufB_p1	ACTTGTTGTTGACCCAACCA	CGTTCTTGAAAACGTGAATCG	45.0	42.9	57.8	56.5	1082-1087 bp	1.0	66.0
ccsA-sufB_p2	TTGTTGTTGACCCAACCAGT	CGTTCTTGAAAACGTGAATCG	45.0	42.9	57.8	56.5	1080-1085 bp	1.0	66.0
ccsA-sufB_p3	CTTGTTGTTGACCCAACCAG	CGTTCTTGAAAACGTGAATCG	50.0	42.9	57.1	56.5	1081-1086 bp	1.0	60.8
ccsA-sufB_p4	AGCTACTTGTTGTTGACCCA	CGTTCTTGAAAACGTGAATCG	45.0	42.9	57.0	56.5	1086-1091 bp	1.0	59.6
ccsA-sufB_p5	TTGTTGTTGACCCAACCAGT	CACGTAAAGGTTTAACACATCGT	45.0	39.1	57.8	57.7	1100-1105 bp	1.0	59.2