Hotspot

rpl21-eboF

LSC · 48459-48913 · 455 bp

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Pi 0.0462

MarkerSeek score 70.40

Primer available yes

Label rpl21-eboF

Feature Metadata

label_name: rpl21-eboF
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 21
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21-eboF_p1	TCAACCCAAAATTGTCTACCGA	ATCATGCAACATTTAAAAGATTAG	40.9	25.0	57.9	52.4	532 bp	1.0	43.2
rpl21-eboF_p2	TCGGTTCAACCCAAAATTGTC	ATCATGCAACATTTAAAAGATTAG	42.9	25.0	57.3	52.4	537 bp	1.0	43.1
rpl21-eboF_p3	CGGTTCAACCCAAAATTGTCT	ATCATGCAACATTTAAAAGATTAG	42.9	25.0	57.3	52.4	536 bp	1.0	43.1
rpl21-eboF_p4	TCGGTTCAACCCAAAATTGT	ATCATGCAACATTTAAAAGATTAG	40.0	25.0	56.1	52.4	537 bp	1.0	43.1
rpl21-eboF_p5	TTCGGTTCAACCCAAAATTGTC	ATCATGCAACATTTAAAAGATTAG	40.9	25.0	58.0	52.4	538 bp	1.0	43.1