Hotspot

trnR(ccg)-trnP(tgg)

SSC · 37341-37534 · 194 bp

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Pi 0.0928

MarkerSeek score 78.30

Primer available yes

Label trnR(ccg)-trnP(tgg)

Feature Metadata

label_name: trnR(ccg)-trnP(tgg)
feature_type: igs
region: SSC
length_bp: 194
variable_sites: 18
pi: 0.0928
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR(ccg)-trnP(tgg)_p1	TGAACCCATACTCTCTGGCA	TACCAAACTGCGCTACATCC	50.0	50.0	58.0	58.0	273-276 bp	1.0	84.7
trnR(ccg)-trnP(tgg)_p2	CTCTGGCATCGGAAACCATT	TACCAAACTGCGCTACATCC	50.0	50.0	58.2	58.0	261-264 bp	1.0	83.4
trnR(ccg)-trnP(tgg)_p3	TGAACCCATACTCTCTGGCA	CTGTGACATTCTGCTCCCAA	50.0	50.0	58.0	57.8	304-307 bp	1.0	82.8
trnR(ccg)-trnP(tgg)_p4	TGAACCCATACTCTCTGGCA	AACCTGTGACATTCTGCTCC	50.0	50.0	58.0	57.8	307-310 bp	1.0	82.7
trnR(ccg)-trnP(tgg)_p5	AAAACCACATAACAGCCCCA	TACCAAACTGCGCTACATCC	45.0	50.0	57.6	58.0	311-314 bp	1.0	81.3