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cbbX-trnM(cat)

SSC · 4026-4161 · 136 bp

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Pi 0.0368

MarkerSeek score 73.60

Primer available yes

Label cbbX-trnM(cat)

Feature Metadata

label_name: cbbX-trnM(cat)
feature_type: igs
region: SSC
length_bp: 136
variable_sites: 5
pi: 0.0368
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cbbX-trnM(cat)_p1	TGTGTAAACTTTCCTCTATTTTGT	CGACCTTGGGCTTATGAGTC	29.2	55.0	54.7	58.1	258 bp	1.0	41.6
cbbX-trnM(cat)_p2	TCTTCATCAAGTTTGTGTAAAC	CGACCTTGGGCTTATGAGTC	31.8	55.0	52.9	58.1	271 bp	1.0	41.5
cbbX-trnM(cat)_p3	TCTTCATCAAGTTTGTGTAAAC	CTTTGGCTGGACTTGAACCT	31.8	50.0	52.9	58.0	292 bp	1.0	41.4
cbbX-trnM(cat)_p4	TGTGTAAACTTTCCTCTATTTTGT	CTTTGGCTGGACTTGAACCT	29.2	50.0	54.7	58.0	279 bp	1.0	41.4
cbbX-trnM(cat)_p5	TCTTCATCAAGTTTGTGTAAAC	TTTGGCTGGACTTGAACCTG	31.8	50.0	52.9	58.3	291 bp	1.0	41.4