Hotspot

ndhE

SSC · 7257-7562 · 306 bp

Back to Viridantha

Pi 0.00436

MarkerSeek score 47.20

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 306
variable_sites: 2
pi: 0.00436
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CATGCTGCGAGTTGTTTCAG	GACTGCAAGGTCAAACCAGA	50.0	50.0	58.0	58.0	1289-1294 bp	1.0	80.5
ndhE_p2	TTGCTTGGCTTTACATCCGT	GACTGCAAGGTCAAACCAGA	45.0	50.0	58.1	58.0	1172-1177 bp	1.0	80.1
ndhE_p3	ACATCCGTCCCAAGGTATCA	GACTGCAAGGTCAAACCAGA	50.0	50.0	58.1	58.0	1160-1165 bp	1.0	79.9
ndhE_p4	CCAATACATCTGTGGGGCAA	GACTGCAAGGTCAAACCAGA	50.0	50.0	57.9	58.0	1137-1142 bp	1.0	79.8
ndhE_p5	CGAGTTGTTTCAGGCCCTAA	GACTGCAAGGTCAAACCAGA	50.0	50.0	57.8	58.0	1282-1287 bp	1.0	79.4