Hotspot

ccsA

SSC · 3759-4712 · 954 bp

Back to Viridantha

Pi 0.00349

MarkerSeek score 61.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 954
variable_sites: 5
pi: 0.00349
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GAGTGGCGGCATCTTCTAAA	TGGTTCTCAAAAACTCATAAAAACA	50.0	28.0	58.0	56.1	1134 bp	1.0	40.4
ccsA_p2	GAGTGGCGGCATCTTCTAAA	ACTTTCGTTATATATGGGATGATGT	50.0	32.0	58.0	56.0	1108 bp	1.0	40.4
ccsA_p3	GCTAAAGCATCTCGGTTCGA	ACTTTCGTTATATATGGGATGATGT	50.0	32.0	58.1	56.0	1132 bp	1.0	40.4
ccsA_p4	GAGTGGCGGCATCTTCTAAA	ACTCATAAAAACAAACTTTCGT	50.0	27.3	58.0	52.8	1122 bp	1.0	40.4
ccsA_p5	GCTAAAGCATCTCGGTTCGA	TGGTTCTCAAAAACTCATAAAAACA	50.0	28.0	58.1	56.1	1158 bp	1.0	40.3