Hotspot

rps8-rpl14

LSC · 126737-126903 · 167 bp

Back to Viridantha

Pi 0.016

MarkerSeek score 62.70

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 4
pi: 0.016
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGATCGGCAATAGTGTCCCT	GAACTCGAGTTTTTGGTGCG	50.0	50.0	58.2	58.0	279 bp	1.0	79.4
rps8-rpl14_p2	AGATCGGCAATAGTGTCCCT	ACTCGAGTTTTTGGTGCGAT	50.0	45.0	58.2	58.1	277 bp	1.0	78.5
rps8-rpl14_p3	AGATCGGCAATAGTGTCCCT	AACTCGAGTTTTTGGTGCGA	50.0	45.0	58.2	58.3	278 bp	1.0	76.8
rps8-rpl14_p4	AGATCGGCAATAGTGTCCCT	GGAACTCGAGTTTTTGGTGC	50.0	50.0	58.2	57.6	280 bp	1.0	76.3
rps8-rpl14_p5	AGATCGGCAATAGTGTCCCT	CTCGAGTTTTTGGTGCGATC	50.0	50.0	58.2	57.5	276 bp	1.0	75.4