Hotspot

psbE-petL

LSC · 110395-111888 · 1494 bp

Back to Viridantha

Pi 0.00357

MarkerSeek score 61.50

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1494
variable_sites: 8
pi: 0.00357
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1636-1659 bp	1.0	75.0
psbE-petL_p2	CAACCCGCAATGAATAGGGA	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1635-1658 bp	1.0	75.0
psbE-petL_p3	ACTGACGAATAACCAACCCG	AAGCAGCCAGTAGAAAACCA	50.0	45.0	57.9	57.3	1649-1672 bp	1.0	74.7
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCAGCCAGTAGAAAACCAA	50.0	45.0	57.9	57.3	1648-1671 bp	1.0	74.7
psbE-petL_p5	ACGAATAACCAACCCGCAAT	AGCAGCCAGTAGAAAACCAA	45.0	45.0	58.2	57.3	1644-1667 bp	1.0	74.1