Hotspot

psbM-trnD-GUC

LSC · 75080-76106 · 1027 bp

Back to Viridantha

Pi 0.00455

MarkerSeek score 61.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1027
variable_sites: 7
pi: 0.00455
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1152-1155 bp	1.0	62.4
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1156-1159 bp	1.0	61.7
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	CATACACCTCGCTGGGATTG	45.0	55.0	55.8	58.4	1158-1161 bp	1.0	59.5
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.4	58.1	1159-1162 bp	1.0	59.2
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1135-1138 bp	1.0	59.1