Hotspot

trnS-GCU-trnG-UCC

LSC · 52138-53199 · 1062 bp

Back to Viridantha

Pi 0.0116

MarkerSeek score 62.80

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 16
pi: 0.0116
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	GAGAAAGCGGAAAGAGAGGG	AACCGATGGACCCCTTAACT	55.0	50.0	58.0	58.3	1212-1320 bp	1.0	81.2
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AACCGATGGACCCCTTAACT	45.0	50.0	58.0	58.3	1157-1265 bp	1.0	81.2
trnS-GCU-trnG-UCC_p3	GAGAAAGCGGAAAGAGAGGG	AAACCGATGGACCCCTTAAC	55.0	50.0	58.0	57.5	1213-1321 bp	1.0	79.9
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AAACCGATGGACCCCTTAAC	45.0	50.0	58.0	57.5	1158-1266 bp	1.0	79.9
trnS-GCU-trnG-UCC_p5	GAGAAAGCGGAAAGAGAGGG	ACCGATGGACCCCTTAACTA	55.0	50.0	58.0	57.4	1211-1319 bp	1.0	79.4