Hotspot

psaC-ndhE

SSC · 6857-7256 · 400 bp

Back to Viridantha

Pi 0.0152

MarkerSeek score 61.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 400
variable_sites: 9
pi: 0.0152
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTGCTTGGCTTTACATCCGT	TTATAGCCATTGCAGCTGCT	45.0	45.0	58.1	57.6	619-624 bp	1.0	78.2
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TTATAGCCATTGCAGCTGCT	50.0	45.0	58.1	57.6	607-612 bp	1.0	78.0
psaC-ndhE_p3	TTGCTTGGCTTTACATCCGT	TATAGCCATTGCAGCTGCTG	45.0	50.0	58.1	58.4	618-623 bp	1.0	77.9
psaC-ndhE_p4	CCAATACATCTGTGGGGCAA	TTATAGCCATTGCAGCTGCT	50.0	45.0	57.9	57.6	584-589 bp	1.0	77.9
psaC-ndhE_p5	TTGCTTGGCTTTACATCCGT	GTTATAGCCATTGCAGCTGC	45.0	50.0	58.1	57.6	620-625 bp	1.0	77.7