Hotspot

rpl33-rps18

LSC · 69632-69993 · 362 bp

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Pi 0.0612
MarkerSeek score 72.60
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
362
variable_sites
89
pi
0.0612
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.79
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGTCTAGTCG CAAACGCCGACGAAAAGATC 50.0 50.0 55.9 58.1 297-547 bp 1.0 73.9
rpl33-rps18_p2 TCTGTCCCTACTGTCATAGACA CAAACGCCGACGAAAAGATC 45.5 50.0 57.4 58.1 260-510 bp 1.0 70.2
rpl33-rps18_p3 TTCTGTCCCTACTGTCATAGACA CAAACGCCGACGAAAAGATC 43.5 50.0 58.1 58.1 261-511 bp 1.0 66.4
rpl33-rps18_p4 CTGTCCCTACTGTCATAGACA CAAACGCCGACGAAAAGATC 47.6 50.0 55.9 58.1 259-509 bp 1.0 66.2
rpl33-rps18_p5 CGACACAATACGTCTAGTCG TCAATATGATCCCCCGATCCA 50.0 47.6 55.9 58.1 326-576 bp 0.952381 64.4