Hotspot

petA-psbJ

LSC · 64548-65666 · 1119 bp

Back to Viola

Pi 0.0265
MarkerSeek score 71.50
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1119
variable_sites
140
pi
0.0265
unique_haplotype_count
38
primer_available
yes
alignment_reliability
0.98
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 TGAGAAGGTTCAATTGTCCGA CATATTCCGGATTGGGCTCA 42.9 50.0 57.2 57.4 951-1200 bp 1.0 73.4
petA-psbJ_p2 TGAGAAGGTTCAATTGTCCGA TATTCCGGATTGGGCTCATC 42.9 50.0 57.2 57.1 949-1198 bp 1.0 71.0
petA-psbJ_p3 TGAGAAGGTTCAATTGTCCGA TCATATTCCGGATTGGGCTC 42.9 50.0 57.2 57.1 952-1201 bp 1.0 71.0
petA-psbJ_p4 TGAGAAGGTTCAATTGTCCGAA CATATTCCGGATTGGGCTCA 40.9 50.0 57.9 57.4 951-1200 bp 1.0 70.8
petA-psbJ_p5 TTGAGAAGGTTCAATTGTCCGA CATATTCCGGATTGGGCTCA 40.9 50.0 57.9 57.4 952-1201 bp 1.0 70.8