Hotspot

psaI-ycf4

LSC · 61212-61667 · 456 bp

Back to Viola

Pi 0.0205
MarkerSeek score 67.00
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
456
variable_sites
56
pi
0.0205
unique_haplotype_count
31
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GTAGGCTTAGTCTTTCCGGC ACTTGTTTTTCGAGACCCCA 55.0 45.0 58.1 57.3 311-609 bp 1.0 85.7
psaI-ycf4_p2 GTAGGCTTAGTCTTTCCGGC TTGTTTTTCGAGACCCCACT 55.0 45.0 58.1 57.3 309-607 bp 1.0 85.7
psaI-ycf4_p3 GTAGGCTTAGTCTTTCCGGC TGTTCTGATCGCCAACTCAT 55.0 45.0 58.1 57.2 271-569 bp 1.0 85.4
psaI-ycf4_p4 GCAATTGCAATGGCTTCCTT TTGTTTTTCGAGACCCCACT 45.0 45.0 57.9 57.3 291-589 bp 0.97619 84.0
psaI-ycf4_p5 GCAATTGCAATGGCTTCCTT ACTTGTTTTTCGAGACCCCA 45.0 45.0 57.9 57.3 293-591 bp 0.97619 83.9