Hotspot

ndhC-trnV-UAC

LSC · 51853-52715 · 863 bp

Back to Viola

Pi 0.0279
MarkerSeek score 75.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
863
variable_sites
133
pi
0.0279
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGATAAAAGGCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 819-1008 bp 1.0 89.8
ndhC-trnV-UAC_p2 CTCCGGCCCTTTTCTAATGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 838-1027 bp 1.0 87.9
ndhC-trnV-UAC_p3 GGGGATAAAAGGCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 818-1007 bp 1.0 87.0
ndhC-trnV-UAC_p4 GGGGATAAAAGGCCGGAAAT GTTCGAGTCCGTATAGCCCT 50.0 55.0 57.9 58.7 807-996 bp 1.0 85.9
ndhC-trnV-UAC_p5 CTCCGGCCCTTTTCTAATGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 58.3 57.5 837-1026 bp 1.0 85.1