Hotspot

trnE-UUC-trnT-GGU

LSC · 31158-32096 · 939 bp

Back to Viola

Pi 0.0273
MarkerSeek score 76.50
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
939
variable_sites
135
pi
0.0273
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.97
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 AATCCCCGTTACCTCCTTGA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 872-1184 bp 1.0 91.6
trnE-UUC-trnT-GGU_p2 ATCCCCGTTACCTCCTTGAA GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 871-1183 bp 1.0 91.6
trnE-UUC-trnT-GGU_p3 GAAGTCGAATCCCCGTTACC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 879-1191 bp 1.0 91.4
trnE-UUC-trnT-GGU_p4 GGAAGTCGAATCCCCGTTAC GAACCGATGACTTACGCCTT 55.0 50.0 58.1 58.0 880-1192 bp 1.0 91.4
trnE-UUC-trnT-GGU_p5 CCCGATCAACTTCCTTTCGT GAACCGATGACTTACGCCTT 50.0 50.0 57.9 58.0 918-1229 bp 1.0 91.2