Hotspot

trnD-GUC-trnY-GUA

LSC · 30540-30927 · 388 bp

Back to Viola

Pi 0.0321
MarkerSeek score 70.10
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
388
variable_sites
55
pi
0.0321
unique_haplotype_count
29
primer_available
yes
alignment_reliability
0.97
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 GCGGTGCTCTTACCAATTGA TGGCAATATGTCTACGCTGG 50.0 50.0 58.6 57.8 419-554 bp 1.0 85.7
trnD-GUC-trnY-GUA_p2 GCGGTGCTCTTACCAATTGA GGCAATATGTCTACGCTGGT 50.0 50.0 58.6 57.8 418-553 bp 1.0 85.7
trnD-GUC-trnY-GUA_p3 GCGGTGCTCTTACCAATTGA GGGGACGGACTGTAAATTCG 50.0 55.0 58.6 58.4 440-575 bp 1.0 84.8
trnD-GUC-trnY-GUA_p4 GCGGTGCTCTTACCAATTGA GGGACGGACTGTAAATTCGT 50.0 50.0 58.6 57.6 439-574 bp 1.0 84.6
trnD-GUC-trnY-GUA_p5 GCGGTGCTCTTACCAATTGA CGGACTGTAAATTCGTTGGC 50.0 50.0 58.6 57.5 435-570 bp 1.0 83.4