Hotspot

psbM-trnD-GUC

LSC · 29254-30465 · 1212 bp

Back to Viola

Pi 0.0154
MarkerSeek score 67.70
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1212
variable_sites
121
pi
0.0154
unique_haplotype_count
31
primer_available
yes
alignment_reliability
0.98
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGAACTAGAATAAACAGTGCAGT TCAATTGGTAAGAGCACCGC 37.5 50.0 57.9 58.6 1202-1345 bp 1.0 54.2
psbM-trnD-GUC_p2 ACTAGAATAAACAGTGCAGTAGC TCAATTGGTAAGAGCACCGC 39.1 50.0 56.4 58.6 1198-1341 bp 1.0 51.6
psbM-trnD-GUC_p3 GGAACTAGAATAAACAGTGCAGT TCAATTGGTAAGAGCACCGC 39.1 50.0 56.5 58.6 1201-1344 bp 1.0 51.5
psbM-trnD-GUC_p4 AGAATAAACAGTGCAGTAGC TCAATTGGTAAGAGCACCGC 40.0 50.0 53.3 58.6 1195-1338 bp 1.0 51.5
psbM-trnD-GUC_p5 AGGAACTAGAATAAACAGTGCAGT AATTGGTAAGAGCACCGCC 37.5 52.6 57.9 58.1 1200-1343 bp 1.0 51.5