Hotspot

petN-psbM

LSC · 27877-29148 · 1272 bp

Back to Viola

Pi 0.0242
MarkerSeek score 66.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1272
variable_sites
160
pi
0.0242
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.94
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTATGGGGAAGAAGTGGACT TGCTACTGCACTGTTTATTCT 47.6 38.1 57.8 55.2 884-1385 bp 1.0 52.3
petN-psbM_p2 AGTATGGGGAAGAAGTGGACT ACTGCACTGTTTATTCTAGTTCCT 47.6 37.5 57.8 57.9 880-1381 bp 1.0 52.0
petN-psbM_p3 AGTATGGGGAAGAAGTGGAC TGCTACTGCACTGTTTATTCT 50.0 38.1 56.2 55.2 884-1385 bp 1.0 52.0
petN-psbM_p4 GTATGGGGAAGAAGTGGACT TGCTACTGCACTGTTTATTCT 50.0 38.1 56.2 55.2 883-1384 bp 1.0 52.0
petN-psbM_p5 AGTATGGGGAAGAAGTGGACT TGCTACTGCACTGTTTATTCTAGT 47.6 37.5 57.8 58.0 884-1376 bp 0.952381 50.1