Hotspot

rpoB-trnC-GCA

LSC · 26106-27262 · 1157 bp

Back to Viola

Pi 0.0117
MarkerSeek score 66.70
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1157
variable_sites
115
pi
0.0117
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CCCTCATTTCCATCCCCAAG CCGGATTTGAACTGGGGAAA 55.0 50.0 57.9 58.1 1113-1285 bp 1.0 90.2
rpoB-trnC-GCA_p2 CTCATTTCCATCCCCAAGCA CCGGATTTGAACTGGGGAAA 50.0 50.0 57.9 58.1 1111-1283 bp 1.0 89.6
rpoB-trnC-GCA_p3 TCCCTCATTTCCATCCCCAA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.3 58.1 1114-1286 bp 1.0 88.3
rpoB-trnC-GCA_p4 TTCCCTCATTTCCATCCCCA CCGGATTTGAACTGGGGAAA 50.0 50.0 58.3 58.1 1115-1287 bp 1.0 88.3
rpoB-trnC-GCA_p5 CCCTCATTTCCATCCCCAAG AAAAGGATTTGCAGTCCCCC 55.0 50.0 57.9 58.4 1095-1267 bp 1.0 88.1