Hotspot

ycf1

SSC · 129307-135363 · 6057 bp

Back to Vincetoxicum

Pi 0.106

MarkerSeek score 77.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 6057
variable_sites: 2034
pi: 0.106
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ATTAGTTCCCGCCGAAAGTC	TAAGTGGGCCCTATCGCTTA	50.0	50.0	58.0	57.9	910-1165 bp	0.833333	82.2
ycf1_p2	ATTAGTTCCCGCCGAAAGTC	TCACCTTCTAACGGGTCGAT	50.0	50.0	58.0	58.2	872-1127 bp	0.833333	81.6
ycf1_p3	TTTTCGTGCCTATCGCTCAA	TAAGTGGGCCCTATCGCTTA	45.0	50.0	57.9	57.9	1123-1378 bp	0.75	77.4
ycf1_p4	TTTTTCGTGCCTATCGCTCA	TAAGTGGGCCCTATCGCTTA	45.0	50.0	57.9	57.9	1124-1379 bp	0.75	77.4
ycf1_p5	TTCCCACCATACCCTCTTGT	TAAGTGGGCCCTATCGCTTA	50.0	50.0	57.9	57.9	861-1116 bp	0.25	49.3