Hotspot

ndhI

SSC · 124638-125132 · 495 bp

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Pi 0.139

MarkerSeek score 74.60

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 495
variable_sites: 225
pi: 0.139
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	ACCTCCTAAGATCAGACCCG	GCTTTTGGGGGTGGAATTTG	55.0	50.0	57.9	57.8	1644-1707 bp	0.833333	75.9
ndhI_p2	CGCGGCAGCTACAAAATAAG	GCTTTTGGGGGTGGAATTTG	50.0	50.0	57.9	57.8	1758-1821 bp	0.833333	75.5
ndhI_p3	ACCTCCTAAGATCAGACCCG	AATATGGCTTTTGGGGGTGG	55.0	50.0	57.9	58.1	1650-1706 bp	0.75	72.7
ndhI_p4	CGCGGCAGCTACAAAATAAG	AATATGGCTTTTGGGGGTGG	50.0	50.0	57.9	58.1	1764-1820 bp	0.75	72.3
ndhI_p5	ATCAGACCCGATCCCAGAAA	AATATGGCTTTTGGGGGTGG	50.0	50.0	58.1	58.1	1640-1696 bp	0.75	72.3