Hotspot

ndhH

SSC · 127376-128572 · 1197 bp

Back to Vincetoxicum

Pi 0.135
MarkerSeek score 78.40
Primer available yes
Label ndhH

Feature Metadata

label_name
ndhH
feature_type
gene
region
SSC
length_bp
1197
variable_sites
533
pi
0.135
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH_p1 ATAACCGACCATCTCCGCTA CAGTTGGGTATTCGAGAGGC 50.0 55.0 58.0 58.1 1610-1626 bp 0.833333 74.3
ndhH_p2 ATAACCGACCATCTCCGCTA AAGGGGGTCGGTTGAATTTC 50.0 50.0 58.0 58.1 1797-1813 bp 0.833333 74.2
ndhH_p3 ATAACCGACCATCTCCGCTA GAAGGGGGTCGGTTGAATTT 50.0 50.0 58.0 58.1 1798-1814 bp 0.833333 74.2
ndhH_p4 GAAGAATTCCTAAGGGCCCG CAGTTGGGTATTCGAGAGGC 55.0 55.0 58.0 58.1 1538-1554 bp 0.833333 74.2
ndhH_p5 AAATAACCGACCATCTCCGC CAGTTGGGTATTCGAGAGGC 50.0 55.0 58.1 58.1 1612-1628 bp 0.833333 73.9