Hotspot

ccsA

SSC · 119817-120785 · 969 bp

Back to Vincetoxicum

Pi 0.121

MarkerSeek score 71.60

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 387
pi: 0.121
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GATGGGGAGAGGTTTCGAAC	ATGAGTGGCTTTGTTGCTGA	55.0	45.0	58.0	58.0	2270-2323 bp	0.75	72.5
ccsA_p2	AAGATGGGGAGAGGTTTCGA	ATGAGTGGCTTTGTTGCTGA	50.0	45.0	58.0	58.0	2272-2325 bp	0.75	72.3
ccsA_p3	AGATGGGGAGAGGTTTCGAA	ATGAGTGGCTTTGTTGCTGA	50.0	45.0	58.0	58.0	2271-2324 bp	0.75	72.3
ccsA_p4	GATGGGGAGAGGTTTCGAAC	TATCTTGACGAAATGGGCGG	55.0	50.0	58.0	58.1	2363-2416 bp	0.666667	68.4
ccsA_p5	AGATGGGGAGAGGTTTCGAA	TATCTTGACGAAATGGGCGG	50.0	50.0	58.0	58.1	2364-2417 bp	0.666667	68.2