Hotspot

ndhC-trnV-UAC

LSC · 56026-57448 · 1423 bp

Back to Vincetoxicum

Pi 0.0139

MarkerSeek score 75.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1423
variable_sites: 61
pi: 0.0139
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GTCCTTTGTTAATCGGGGCT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1503-1717 bp	1.0	84.5
ndhC-trnV-UAC_p2	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1489-1703 bp	1.0	84.4
ndhC-trnV-UAC_p3	GTCCTTTGTTAATCGGGGCT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1502-1716 bp	1.0	81.8
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1488-1702 bp	1.0	81.7
ndhC-trnV-UAC_p5	TGGTCCTTTGTTAATCGGGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1505-1719 bp	1.0	81.5