Hotspot

rps4-trnT-UGU

LSC · 51373-51822 · 450 bp

Back to Vincetoxicum

Pi 0.0153

MarkerSeek score 69.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 450
variable_sites: 21
pi: 0.0153
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TCCCGAACGAGATTGGTTTC	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	515-643 bp	1.0	85.0
rps4-trnT-UGU_p2	CCCGAACGAGATTGGTTTCT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.9	57.9	514-642 bp	1.0	84.9
rps4-trnT-UGU_p3	TCCCGAACGAGATTGGTTTC	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	483-611 bp	1.0	84.3
rps4-trnT-UGU_p4	GATCATTTCCGGCTCTAGGC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.2	57.9	492-620 bp	1.0	84.2
rps4-trnT-UGU_p5	CCCGAACGAGATTGGTTTCT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.9	57.8	482-610 bp	1.0	84.2