Hotspot

trnQ-UUG-psbK

LSC · 7512-8070 · 559 bp

Back to Vincetoxicum

Pi 0.0164

MarkerSeek score 76.20

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 559
variable_sites: 37
pi: 0.0164
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TGAATATGCTCTGGGACGGA	AAAAAGCGTAGGCCTCGG	50.0	55.6	58.2	57.7	731-889 bp	1.0	67.9
trnQ-UUG-psbK_p2	GACCAAAACCCGTTGCCTTA	AAAAAGCGTAGGCCTCGG	50.0	55.6	58.7	57.7	687-845 bp	1.0	64.4
trnQ-UUG-psbK_p3	ATGAATATGCTCTGGGACGG	AAAAAGCGTAGGCCTCGG	50.0	55.6	57.2	57.7	732-890 bp	1.0	62.8
trnQ-UUG-psbK_p4	GAATATGCTCTGGGACGGAA	AAAAAGCGTAGGCCTCGG	50.0	55.6	57.1	57.7	730-888 bp	1.0	62.3
trnQ-UUG-psbK_p5	AATATGCTCTGGGACGGAAG	AAAAAGCGTAGGCCTCGG	50.0	55.6	57.1	57.7	729-887 bp	1.0	62.2