Hotspot

ndhE-ndhG

SSC · 117754-117863 · 110 bp

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Pi 0.0182
MarkerSeek score 63.00
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
110
variable_sites
2
pi
0.0182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 GACACATAAGGGCTCTAACCA CAATTGCTGTTGCTCGTCAA 47.6 45.0 57.1 57.6 233-234 bp 1.0 62.2
ndhE-ndhG_p2 ACAAGTACATGTTCGAGCATCA CAATTGCTGTTGCTCGTCAA 40.9 45.0 58.1 57.6 156-157 bp 1.0 61.2
ndhE-ndhG_p3 AGTTCAAGACACATAAGGGCTC CAATTGCTGTTGCTCGTCAA 45.5 45.0 58.1 57.6 240-241 bp 1.0 61.0
ndhE-ndhG_p4 GTTCAAGACACATAAGGGCTCT CAATTGCTGTTGCTCGTCAA 45.5 45.0 58.1 57.6 239-240 bp 1.0 61.0
ndhE-ndhG_p5 AGTTCAAGACACATAAGGGCT CAATTGCTGTTGCTCGTCAA 42.9 45.0 56.9 57.6 240-241 bp 1.0 60.8