Hotspot

ccsA-ndhD

SSC · 115093-115334 · 242 bp

Back to Vinca

Pi 0.062
MarkerSeek score 67.70
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
242
variable_sites
15
pi
0.062
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGTTATGGTTCGTTTATATTAACATCT ACCCGGATTTCGTTCTTTCA 25.9 45.0 55.3 57.4 346 bp 1.0 43.7
ccsA-ndhD_p2 AGTTATGGTTCGTTTATATTAACA ACCCGGATTTCGTTCTTTCA 25.0 45.0 52.3 57.4 346 bp 1.0 43.7
ccsA-ndhD_p3 ACATAGTTATGGTTCGTTTATATT ACCCGGATTTCGTTCTTTCA 25.0 45.0 52.1 57.4 350 bp 1.0 43.7
ccsA-ndhD_p4 AGTTATGGTTCGTTTATATTAACATC ACCCGGATTTCGTTCTTTCA 26.9 45.0 54.1 57.4 346 bp 1.0 43.7
ccsA-ndhD_p5 GTTATGGTTCGTTTATATTAACATCT ACCCGGATTTCGTTCTTTCA 26.9 45.0 54.1 57.4 345 bp 1.0 43.7