Hotspot

ycf1-ndhF

SSC · 110385-110400 · 16 bp

Back to Vinca

Pi 0.625
MarkerSeek score 72.50
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
SSC
length_bp
16
variable_sites
10
pi
0.625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 CAACTTCCGAGATGAAGGGG TCCTTTCAATCAAGCGGGAG 55.0 50.0 58.0 57.9 787-818 bp 1.0 82.1
ycf1-ndhF_p2 AACTTCCGAGATGAAGGGGA TCCTTTCAATCAAGCGGGAG 50.0 50.0 58.0 57.9 786-817 bp 1.0 82.0
ycf1-ndhF_p3 CAACTTCCGAGATGAAGGGG CCTTTCAATCAAGCGGGAGT 55.0 50.0 58.0 58.2 786-817 bp 1.0 81.7
ycf1-ndhF_p4 AACTTCCGAGATGAAGGGGA CCTTTCAATCAAGCGGGAGT 50.0 50.0 58.0 58.2 785-816 bp 1.0 81.5
ycf1-ndhF_p5 AGGGTGGAAAGTGAGGAAGA TCCTTTCAATCAAGCGGGAG 50.0 50.0 57.9 57.9 827-858 bp 1.0 81.3