Hotspot

ycf15-trnL-CAA

IRb · 94202-94602 · 401 bp

Back to Vinca

Pi 0.015
MarkerSeek score 63.20
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
401
variable_sites
6
pi
0.015
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 TGAACAGCGAACAACCAGTT AAATGGTAGACACGCGAGAC 45.0 50.0 58.3 58.0 538 bp 1.0 78.8
ycf15-trnL-CAA_p2 TGAACAGCGAACAACCAGTT TGAAATGGTAGACACGCGAG 45.0 50.0 58.3 58.0 540 bp 1.0 78.8
ycf15-trnL-CAA_p3 TGAACAGCGAACAACCAGTT GCCTTGGTGGTGAAATGGTA 45.0 50.0 58.3 58.1 550 bp 1.0 78.2
ycf15-trnL-CAA_p4 TGAACAGCGAACAACCAGTT TGGTGAAATGGTAGACACGC 45.0 50.0 58.3 58.2 543 bp 1.0 77.3
ycf15-trnL-CAA_p5 TGAACAGCGAACAACCAGTT GAAATGGTAGACACGCGAGA 45.0 50.0 58.3 57.7 539 bp 1.0 76.7