Hotspot

psbM-trnD-GUC

LSC · 29546-30402 · 857 bp

Back to Vinca

Pi 0.0246
MarkerSeek score 63.90
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
857
variable_sites
21
pi
0.0246
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 982-991 bp 1.0 69.9
psbM-trnD-GUC_p2 AGAAAGGCGGTAGGAACTAGA TTCAATTGGTCAGAGCACCG 47.6 50.0 57.9 58.5 986-995 bp 1.0 69.3
psbM-trnD-GUC_p3 AGTAGAAAGGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 47.6 50.0 58.2 58.5 989-998 bp 1.0 68.7
psbM-trnD-GUC_p4 AGTAGAAAGGCGGTAGGAAC TTCAATTGGTCAGAGCACCG 50.0 50.0 56.7 58.5 989-998 bp 1.0 67.4
psbM-trnD-GUC_p5 GTAGAAAGGCGGTAGGAACT TTCAATTGGTCAGAGCACCG 50.0 50.0 56.7 58.5 988-997 bp 1.0 67.4