Hotspot

ycf1

SSC · 120717-125888 · 5172 bp

Pi 0.0175

MarkerSeek score 53.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5172
variable_sites: 135
pi: 0.0175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGAACTATTCCTTGTGCTCCA	AACGGCGTTCAATTATCCGA	42.9	45.0	56.9	58.0	1202-1208 bp	1.0	65.9
ycf1_p2	TGAACTATTCCTTGTGCTCCA	ACGGCGTTCAATTATCCGAA	42.9	45.0	56.9	58.0	1201-1207 bp	1.0	65.9
ycf1_p3	TGTTTTCGCTCAATTCATCGAG	ACGGCGTTCAATTATCCGAA	40.9	45.0	57.7	58.0	489 bp	1.0	64.7
ycf1_p4	TGGTTGTGACAAAAAGGAGACA	AACGGCGTTCAATTATCCGA	40.9	45.0	58.3	58.0	1059-1065 bp	1.0	63.8
ycf1_p5	TGGTTGTGACAAAAAGGAGACA	ACGGCGTTCAATTATCCGAA	40.9	45.0	58.3	58.0	1058-1064 bp	1.0	63.8