Hotspot

rpoC1

LSC · 40520-43422 · 2903 bp

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Pi 0.00854
MarkerSeek score 50.20
Primer available yes
Label rpoC1

Feature Metadata

label_name
rpoC1
feature_type
gene
region
LSC
length_bp
2903
variable_sites
37
pi
0.00854
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoC1_p1 AGGAAGGAAGCTTAATTGGACT TGTTTATCTTAGATACAACCATCT 40.9 29.2 57.1 53.3 2954-2974 bp 1.0 41.6
rpoC1_p2 GGAAGGAAGCTTAATTGGACTG TGTTTATCTTAGATACAACCATCT 45.5 29.2 57.0 53.3 2953-2973 bp 1.0 41.6
rpoC1_p3 AGGAAGGAAGCTTAATTGGACT TTGTTTATCTTAGATACAACCATCT 40.9 28.0 57.1 54.0 2955-2975 bp 1.0 41.6
rpoC1_p4 GGAAGGAAGCTTAATTGGACTG TTGTTTATCTTAGATACAACCATCT 45.5 28.0 57.0 54.0 2954-2974 bp 1.0 41.6
rpoC1_p5 AGGAAGGAAGCTTAATTGGACTG TGTTTATCTTAGATACAACCATCT 43.5 29.2 58.4 53.3 2954-2974 bp 1.0 41.6