Hotspot

ndhF

SSC · 107943-110192 · 2250 bp

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Pi 0.0141
MarkerSeek score 52.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2250
variable_sites
47
pi
0.0141
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AGCAGCAACAACTGGGTTTA TGATCTAGACGTGCGCTTTT 45.0 45.0 57.9 57.6 2980 bp 1.0 78.3
ndhF_p2 AGCAGCAACAACTGGGTTTA TTGATCTAGACGTGCGCTTT 45.0 45.0 57.9 57.6 2981 bp 1.0 78.3
ndhF_p3 AGCAGCAACAACTGGGTTTA TTTGATCTAGACGTGCGCTT 45.0 45.0 57.9 57.6 2982 bp 1.0 78.3
ndhF_p4 AGCAGCAACAACTGGGTTTA TTTTGATCTAGACGTGCGCT 45.0 45.0 57.9 57.6 2983 bp 1.0 78.3
ndhF_p5 TAGCAGCAACAACTGGGTTT TGATCTAGACGTGCGCTTTT 45.0 45.0 57.9 57.6 2981 bp 1.0 78.3