Hotspot

rps7-rps12

IRb · 95947-96000 · 54 bp

Back to Vigna

Pi 0.0247
MarkerSeek score 67.60
Primer available yes
Label rps7-rps12

Feature Metadata

label_name
rps7-rps12
feature_type
igs
region
IRb
length_bp
54
variable_sites
2
pi
0.0247
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps7-rps12_p1 TTTCTTCTGCAGTACCTCGC CCACCCTACAATATGGTGCC 50.0 55.0 57.9 58.0 117 bp 1.0 80.6
rps7-rps12_p2 TTTCTTCTGCAGTACCTCGC CTGTGGAAAGCCGTATTCGA 50.0 50.0 57.9 58.0 187 bp 1.0 80.2
rps7-rps12_p3 TTTCTTCTGCAGTACCTCGC TCTGTGGAAAGCCGTATTCG 50.0 50.0 57.9 58.0 188 bp 1.0 80.2
rps7-rps12_p4 ATTGGATCGGATTTTGCGGT CTGTGGAAAGCCGTATTCGA 45.0 50.0 58.2 58.0 209 bp 1.0 78.8
rps7-rps12_p5 ATTGGATCGGATTTTGCGGT TCTGTGGAAAGCCGTATTCG 45.0 50.0 58.2 58.0 210 bp 1.0 78.8