Hotspot

psbK-trnQ-UUG

LSC · 57070-57519 · 450 bp

Back to Vigna

Pi 0.026
MarkerSeek score 65.00
Primer available yes
Label psbK-trnQ-UUG

Feature Metadata

label_name
psbK-trnQ-UUG
feature_type
igs
region
LSC
length_bp
450
variable_sites
17
pi
0.026
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-trnQ-UUG_p1 TCGGGCAATTTCTCCAACAA AGATATGCTCTGGGACGGAA 45.0 50.0 58.0 57.9 611-614 bp 1.0 81.8
psbK-trnQ-UUG_p2 TTCGGGCAATTTCTCCAACA AGATATGCTCTGGGACGGAA 45.0 50.0 58.0 57.9 612-615 bp 1.0 81.8
psbK-trnQ-UUG_p3 TAGGCTTCGGGCAATTTCTC AGATATGCTCTGGGACGGAA 50.0 50.0 58.0 57.9 617-620 bp 1.0 81.6
psbK-trnQ-UUG_p4 TGGAATAAAGGACGGAGGGA AGATATGCTCTGGGACGGAA 50.0 50.0 57.7 57.9 583-586 bp 1.0 80.0
psbK-trnQ-UUG_p5 GTAGGCTTCGGGCAATTTCT AGATATGCTCTGGGACGGAA 50.0 50.0 58.2 57.9 618-621 bp 1.0 79.9