Hotspot

atpI-atpH

LSC · 49700-50708 · 1009 bp

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Pi 0.0211
MarkerSeek score 76.70
Primer available yes
Label atpI-atpH

Feature Metadata

label_name
atpI-atpH
feature_type
igs
region
LSC
length_bp
1009
variable_sites
32
pi
0.0211
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI-atpH_p1 GGTGAATCCATTGAAGGCCA CAGCAGCAATAACAGAAGCG 50.0 50.0 58.1 57.8 1037-1077 bp 1.0 82.8
atpI-atpH_p2 AGGTGAATCCATTGAAGGCC CAGCAGCAATAACAGAAGCG 50.0 50.0 57.9 57.8 1038-1078 bp 1.0 82.8
atpI-atpH_p3 GGTGAATCCATTGAAGGCCA GCCAATCCAGCAGCAATAAC 50.0 50.0 58.1 57.8 1044-1084 bp 1.0 82.4
atpI-atpH_p4 AGGTGAATCCATTGAAGGCC GCCAATCCAGCAGCAATAAC 50.0 50.0 57.9 57.8 1045-1085 bp 1.0 82.4
atpI-atpH_p5 GGTGAATCCATTGAAGGCCA CAATCCCCTCTACAGCTTGC 50.0 55.0 58.1 58.3 1115-1155 bp 1.0 81.2