Hotspot

psbD-trnT-GGU

LSC · 31256-32425 · 1170 bp

Back to Vigna

Pi 0.03
MarkerSeek score 69.90
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1170
variable_sites
52
pi
0.03
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CCGCAACCAGTCATCCATAA GTGGTAGAGTAACGCCATGG 50.0 55.0 58.0 58.1 1285-1300 bp 1.0 83.3
psbD-trnT-GGU_p2 CCGCAACCAGTCATCCATAA TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.0 58.1 1288-1303 bp 1.0 83.2
psbD-trnT-GGU_p3 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1306-1321 bp 1.0 82.2
psbD-trnT-GGU_p4 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1307-1322 bp 1.0 82.2
psbD-trnT-GGU_p5 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1308-1323 bp 1.0 82.2