Hotspot

trnS-UGA-psbC

LSC · 28621-28824 · 204 bp

Back to Vigna

Pi 0.0817
MarkerSeek score 78.20
Primer available yes
Label trnS-UGA-psbC

Feature Metadata

label_name
trnS-UGA-psbC
feature_type
igs
region
LSC
length_bp
204
variable_sites
22
pi
0.0817
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbC_p1 GAGAGATGGCTGAGTGGTTG ATTTGCGTGCTCCTTGGTTA 55.0 45.0 58.0 58.1 631-640 bp 1.0 83.5
trnS-UGA-psbC_p2 TGATCCCTCAACCCCTTGAT ATTTGCGTGCTCCTTGGTTA 50.0 45.0 58.0 58.1 950-960 bp 1.0 82.9
trnS-UGA-psbC_p3 TGATCCCTCAACCCCTTGAT GAACCTCTAAGGGGCCCTAA 50.0 55.0 58.0 58.1 930-940 bp 1.0 82.8
trnS-UGA-psbC_p4 GAGAGATGGCTGAGTGGTTG CACTGGTCCAGAAGCTTCTC 55.0 55.0 58.0 57.9 798-807 bp 1.0 82.6
trnS-UGA-psbC_p5 TGATCCCTCAACCCCTTGAT CACTGGTCCAGAAGCTTCTC 50.0 55.0 58.0 57.9 1117-1127 bp 1.0 82.4